What is AlphaScreen?

AlphaScreen™ is a bead-based chemistry used to study biomolecular interac- tions in a microplate format. The acronym ALPHA stands for Amplified Luminescent Proximity Homogeneous Assay. As the name implies, some of the key features are that it is a non-radioactive, homogeneous proximity assay.

What is AlphaScreen assay?

AlphaScreen® (ALPHA for Amplified Luminescent Proximity Homogeneous Assay) is a bead-based chemistry used to study interactions between molecules in a microplate. The fundamental principle of the AlphaScreen technology relies on binding two different molecules of interest to specific beads.

What is an AlphaLISA?

AlphaLISA is a bead-based, no-wash luminescent technology. When Alpha Donor and Acceptor beads are brought together through binding to the target, a cascade of chemical reactions is set in motion, creating an amplified signal.

What is Htrf?

HTRF (Homogeneous Time Resolved Fluorescence) is the most frequently used generic assay technology to measure analytes in a homogenous format, which is the ideal platform used for drug target studies in high-throughput screening (HTS).

What is NanoBRET?

The NanoBRET™ assay is a bioluminescence resonance energy transfer (BRET)-based assay that uses NanoLuc® Luciferase as the BRET energy donor and HaloTag® protein labeled with the HaloTag® NanoBRET™ 618 fluorescent Ligand as the energy acceptor to measure the interaction of two binding partners in live cells.

What is fluorescence polarization assay?

Fluorescence polarization (FP) technology is based on the measurement of molecule rotation, and has been widely used to study molecular interactions in solution. This method can be used to measure binding and dissociation between two molecules if one of the binding molecules is relatively small and fluorescent.

What is gyrolab?

Gyrolab® technology delivers high-quality immunoassay data with broad dynamic ranges that enables you to save time (assay time and manual, hands-on time), sample and reagents. Using an affinity flow-through format, Gyrolab technology simplifies assay workflows by eliminating incubations and shortening run times.

What is delfia assay?

Assay principle DELFIA® (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. Assays are designed to detect the presence of a compound or biomolecule using lanthanide chelate labeled reagents, separating unbound reagent using wash steps.

Is Htrf the same as TR-FRET?

HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. HTRF assays require a microplate reader with TRF detection mode that is certified HTRF-compatible by Cisbio.

What is TR-FRET assay?

TR-FRET combines the low background aspect of TRF with the homogeneous assay format of FRET. The resulting assay provides an increase in flexibility, reliability and sensitivity in addition to higher throughput and fewer false positive/false negative results. FRET involves two fluorophores, a donor and an acceptor.

What is BRET ratio?

BRET ratio is defined as the ratio of acceptor and donor bioluminescence emission (A/D) from which A/D obtained with donor alone (RLuc8 or RLuc8. The bioluminescence spectra illustrate the emission spectra of the RLuc mutants and the red fluorescent acceptor proteins.

How do BRET assays work?

BRET, or Bioluminescence Resonance Energy Transfer, is a cell-based assay for studying protein-protein interactions. BRET relies on Forster resonance energy transfer from a bioluminescent donor to an acceptor in the presence of a substrate. Interestingly, BRET is a naturally occurring phenomenon in sea pansy.

Which is more flexible TR-FRET or alphascreen?

The AlphaScreen and AlphaLISA assay platform is much more flexible than TR-FRET. The AlphaScreen and AlphaLISA assay platform can allow for detection of analytes ranging in size from small molecules to large viral particles, with a maximum distance of 200 nm between the Donor and Acceptor beads.

What is the difference between Alpha screen and alphalisa?

Principle of the AlphaLISA assay technology AlphaScreen ® and AlphaLISA ® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym “Alpha” stands for amplified luminescent proximity homogeneous assay.

What’s the difference between TR-FRET and alphalisa?

Proximity assay with broad energy transfer distance (distance between Donor and Acceptor beads can be up to 200 nm or more, as compared to TR-FRET which has a maximum transfer distance of ~10 nm) Compatible with complex matrices such as cell lysates, serum, plasma, CSF, etc. (AlphaLISA)

What was the original purpose of Alpha screen?

Originally developed as a methodology for diagnostic assays called LOCI ® (Luminescent Oxygen Channeling Immunoassay) in 1994 1, today the AlphaScreen Technology (Alpha Technology) includes AlphaScreen, AlphaLISA ® and AlphaPlex TM. It is mainly used in the life sciences for screening purposes.