What does fetal bovine serum contain?

Fetal bovine serum (FBS) is the liquid fraction of clotted blood from fetal calves, depleted of cells, fibrin and clotting factors, but containing a large number of nutritional and macromolecular factors essential for cell growth.

What is bovine serum made of?

Fetal bovine serum (FBS) is a common component of animal cell culture media. It is harvested from bovine fetuses taken from pregnant cows during slaughter. FBS is commonly harvested by means of a cardiac puncture without any form of anaesthesia.

What proteins are in fetal bovine serum?

The globular protein, bovine serum albumin (BSA), is a major component of fetal bovine serum. The rich variety of proteins in fetal bovine serum maintains cultured cells in a medium in which they can survive, grow, and divide.

What is fetal bovine serum used for?

Fetal bovine serum (FBS) is a byproduct of harvesting cattle for the meatpacking industry—it’s used extensively by both academic and industrial researchers as a supplement to basal growth medium in cell culture applications. FBS is the liquid portion that remains after blood is drawn from bovine fetus coagulates.

Is fetal bovine serum safe?

Out of all bovine blood products, Fetal Bovine Serum is considered the safest one since sourced from an unborn animal.

What is the disadvantage of serum?

The disadvantages of serum are described, including variability, shelf life, availability, effect on down-stream processing, and potential for contamination.

What is bovine blood used for?

Animal Blood in Other Applications

Blood source Sector Function/use
Bovine thrombin Medical Promotes blood coagulation
Treatment of wounds
Hold skin grafts in place
Bovine plasma Microbiology Medium for the growth of probiotic bacteria (Lactobacillus sp.)

How much is fetal bovine serum?

Bovine Serum

Item# Item Desc Price
SER-100 Fetal Bovine Serum, Stem Cell Qualified, US Origin $133.00
SER-500 Fetal Bovine Serum, Stem Cell Qualified, US Origin $452.00
SER-HI-500 Fetal Bovine Serum, Stem Cell Qualified, US Origin (HEAT INACTIVATED) $462.00
SERCS-100 Calf Serum, Iron Supplemented $36.00

How do you replace fetal bovine serum?

SummaryObjective: The need for an alternative to fetal bovine serum (FBS) is known to scientists and users involved in cell therapy or advanced therapy medicinal products. Human serum (huS) and platelet lysate (hPL) can be used as alternatives resulting in similar or even superior results concerning cell expansion.

Is DMEM serum-free?

Use a base medium that supports serum-free growth. DMEM/F12 is a good base medium for most cell lines. The F12 portion contains many of the micro-nutrients that cells need for serum-free growth.

Is RPMI serum-free?

RPMI 1640 has traditionally been used for the serum-free expansion of human lymphoid cells. RPMI 1640 uses a bicarbonate buffering system and differs from most mammalian cell culture media in its typical pH 8 formulation.

What is bovine blood?

Bovine blood is made up of 80.9% water, 17.3% protein, 0.23% fat, 0.07% carbohydrate, and 0.62% minerals (Alencar, 1983). Blood composition is similar to meat composition except for the iron (36.3 mg/100 g of blood), which is ∼10 times the concentration in meat (Wismer-Pedersen, 1979; Alencar, 1983).

What do you need to know about fetal bovine serum?

Fetal Bovine Serum. Fetal bovine serum (FBS) is the liquid fraction of clotted blood from fetal calves, depleted of cells, fibrin and clotting factors, but containing a large number of nutritional and macromolecular factors essential for cell growth. Bovine serum albumin is the major component of FBS.

What are the different types of bovine serum?

Standard bovine definitions Terms Definitions Serum Serum is the liquid fraction of clotted Fetal Bovine Serum Semi-processed Fetal Bovine Serum Fetal Specialty Fetal Bovine Serum This is semi-processed FBS or sterile fi Newborn Calf Serum Newborn Calf Serum (NBCS) is defined as

Where does bovine serum from a calf come from?

Fetal bovine serum is sourced from fetuses. Newborn bovine serum is sourced from calves less than twenty days of age. Calf bovine serum is sourced from calves aged between three weeks to 12 months. Adult bovine serum is sourced from cows more than 12 months old.

How is heat inactivated in fetal bovine serum?

Dialyzed— removes any molecule less than 10,000 MW, including antibiotics, amino acids, cytokines, glucose, and hormones. Heat inactivation— by placing FBS in a water bath at 56 degrees Celsius for thirty minutes, inhibitors are deactivated.